4ATH
MITF apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.375, 39.891, 55.160 |
| Unit cell angles | 90.00, 117.28, 90.00 |
Refinement procedure
| Resolution | 49.030 - 1.950 |
| R-factor | 0.19566 |
| Rwork | 0.194 |
| R-free | 0.22778 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.148 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.130 | 0.540 |
| Number of reflections | 14742 | |
| <I/σ(I)> | 9.2 | 3.2 |
| Completeness [%] | 96.2 | 86.8 |
| Redundancy | 6.9 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.5 M AMMONIUM SULFATE AND 150 MM SODIUM ACETATE (PH 5.0) |
