4ATD
Crystal structure of native Raucaffricine glucosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-11 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 102.237, 127.253, 216.111 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.100 |
| R-factor | 0.17524 |
| Rwork | 0.175 |
| R-free | 0.20588 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jf6 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.853 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.110 | 0.730 |
| Number of reflections | 81144 | |
| <I/σ(I)> | 28.4 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.2 | 15.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
