4AT8
Crystal structure of the NF90-NF45 dimerisation domain complex with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-15 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.871, 84.128, 159.597 |
Unit cell angles | 90.00, 93.34, 90.00 |
Refinement procedure
Resolution | 57.844 - 2.692 |
R-factor | 0.2409 |
Rwork | 0.240 |
R-free | 0.26560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4at7 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.647 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.000 | 2.840 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.100 | 0.380 |
Number of reflections | 50589 | |
<I/σ(I)> | 8 | 2.9 |
Completeness [%] | 97.1 | 93.7 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 12-14 % PEG3350, 200 MM MGCL2, 100 MM MES PH 6.5, 5 % GLYCEROL |