4AT7
Crystal structure of the NF90-NF45 dimerisation domain complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-05-10 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 82.900, 131.300, 176.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 88.000 - 1.902 |
R-factor | 0.1863 |
Rwork | 0.185 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | INITIAL MODEL GENERATED FROM A GOLD DERIVATIVE SOLVED IN SPACE GROUP C 2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.035 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.080 | 0.600 |
Number of reflections | 74599 | |
<I/σ(I)> | 15.3 | 3.4 |
Completeness [%] | 98.4 | 95.4 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 100 MM MES PH 6.0, 0.22 M SODIUM MALONATE, 18 % PEG 8000 |