4AT1

STRUCTURAL CONSEQUENCES OF EFFECTOR BINDING TO THE T STATE OF ASPARTATE CARBAMOYLTRANSFERASE. CRYSTAL STRUCTURES OF THE UNLIGATED AND ATP-, AND CTP-COMPLEXED ENZYMES AT 2.6-ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 3 2 1
Unit cell lengths	122.000, 122.000, 142.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.600
R-factor	0.17 *
Rwork	0.160
RMSD bond length	0.014 *
RMSD bond angle	3.400 *
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.600 *
Rmerge	0.046 *
Total number of observations	74676 *
Number of reflections	23826 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	5.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	maleic acid	100 (mM)
2	1	1	Tris-HCl	10 (mM)
3	1	1	2-mercaptoethanol	1 (mM)
4	1	1	EDTA	0.2 (mM)
5	1	1		0.3 (mM)
7	1	2	protein	6 (mg/ml)