4ASU
F1-ATPase in which all three catalytic sites contain bound nucleotide, with magnesium ion released in the Empty site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.268, 135.113, 266.355 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.500 - 2.600 |
| R-factor | 0.24711 |
| Rwork | 0.245 |
| R-free | 0.28933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jdi |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.892 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 133.180 | 2.730 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.120 | 0.840 |
| Number of reflections | 92081 | |
| <I/σ(I)> | 4.4 | 1.7 |
| Completeness [%] | 78.4 | 66 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
