4ARY
Lepidopteran-specific toxin Cry1Ac in complex with receptor specificity determinant GalNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-22 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 121.360, 51.190, 210.650 |
| Unit cell angles | 90.00, 105.75, 90.00 |
Refinement procedure
| Resolution | 223.610 - 2.950 |
| R-factor | 0.21669 |
| Rwork | 0.215 |
| R-free | 0.24433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHAIN A OF PDB ENTRY 4ARX |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.232 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.890 | 3.110 |
| High resolution limit [Å] | 2.350 | 2.950 |
| Rmerge | 0.140 | 0.300 |
| Number of reflections | 46706 | |
| <I/σ(I)> | 8 | 2.2 |
| Completeness [%] | 87.1 | 59.7 |
| Redundancy | 3.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 9.8 | MICRODIALYSIS AGAINST 68-70 MM REMARK 280 PIPERAZINE PH 9.8, 28-30 MM NABR, 20-22% GLYCEROL, 9% MPD, 0.5% GALNAC. FOR DETAILS SEE REFERENCE IN REMARK 1 |
