4ARX
Lepidoptera-specific toxin Cry1Ac from Bacillus thuringiensis ssp. kurstaki HD-73
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.808, 112.975, 123.299 |
| Unit cell angles | 113.11, 91.52, 100.51 |
Refinement procedure
| Resolution | 112.510 - 2.350 |
| R-factor | 0.18552 |
| Rwork | 0.184 |
| R-free | 0.21772 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ciy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.221 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.190 | 2.470 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.100 | 0.220 |
| Number of reflections | 95755 | |
| <I/σ(I)> | 11.2 | 3.7 |
| Completeness [%] | 90.4 | 75.1 |
| Redundancy | 2.4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 9.8 | MICRODIALYSIS AGAINST 65-69 MM PIPERAZINE PH 9.8, 25-29 MM NABR, 21-25% GLYCEROL, 9% MPD, 1% PEG350MME. FOR DETAILS SEE REFERNCE 1 IN REMARK 1 |
