4ART

STRUCTURE OF THE ORF273 PROTEIN FROM THE ACIDIANUS TWO-TAILED VIRUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	110
Detector technology	PIXEL
Collection date	2010-07-25
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 41 21 2
Unit cell lengths	78.620, 78.620, 189.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.420 - 2.150
R-factor	0.2041
Rwork	0.202
R-free	0.23380
Structure solution method	SAD
Starting model (for MR)	NONE
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	autoSHARP
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.210
High resolution limit [Å]	2.150	2.150
Rmerge	0.080	0.740
Number of reflections	32549
<I/σ(I)>	24	3.72
Completeness [%]	97.9	99.9
Redundancy	12.8	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		PROTEIN SOLUTION AT 5 MG/ML IN 10 MM BICINE PH 8.5 AND 100 MM NACL WAS MIXED IN A 3:1 RATIO TO A SOLUTION CONTAINING 3.6% ISOPROPANOL AND 1.9 M AMMONIUM SULPHATE AS PRECIPITANT AGENTS IN 5 MM MGCL2 AND 2 MM AMP
1		6		PROTEIN SOLUTION AT 5 MG/ML IN 10 MM BICINE PH 8.5 AND 100 MM NACL WAS MIXED IN A 3:1 RATIO TO A SOLUTION CONTAINING 3.6% ISOPROPANOL AND 1.9 M AMMONIUM SULPHATE AS PRECIPITANT AGENTS IN 5 MM MGCL2 AND 2 MM AMP