4ARH
X ray structure of the periplasmic zinc binding protein ZinT from Salmonella enterica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 58.534, 58.534, 289.316 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.690 - 2.300 |
| R-factor | 0.22388 |
| Rwork | 0.221 |
| R-free | 0.28841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 10EK |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.283 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.110 | 0.240 |
| Number of reflections | 14007 | |
| <I/σ(I)> | 25.62 | 14.75 |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 12.7 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | AMMONIUM SULFATE 2 M, SODIUM ACETATE 0.1 M PH 5 |
