4AR9
Crystal structure of the peptidase domain of collagenase T from Clostridium tetani at 1.69 angstrom resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.890, 102.110, 104.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.450 - 1.690 |
| R-factor | 0.17482 |
| Rwork | 0.172 |
| R-free | 0.22105 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y3u |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.393 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.850 | 1.780 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.100 | 0.800 |
| Number of reflections | 88975 | |
| <I/σ(I)> | 8.7 | 2 |
| Completeness [%] | 96.2 | 94.5 |
| Redundancy | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 25% PEG 3350, 100MM NACL, 0.1M MES PH 6.5 |
