4AQW
Model of human kinesin-5 motor domain (1II6, 3HQD) and mammalian tubulin heterodimer (1JFF) docked into the 9.5-angstrom cryo-EM map of microtubule-bound kinesin-5 motor domain in the rigor state.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
EM Data Collection
Microscope | FEI TECNAI F20 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2 |
Detector | KODAK SO-163 FILM |
Number of Images | 138 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 9.5 |
Software | Flex-EM,UCSF Chimera,FREALIGN,SPIDER |