4AQP
The structure of the AXH domain of ataxin-1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.539, 82.699, 137.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.916 - 2.452 |
| R-factor | 0.2201 |
| Rwork | 0.217 |
| R-free | 0.26720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oa8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.723 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.900 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.100 | 0.700 |
| Number of reflections | 16989 | |
| <I/σ(I)> | 14.8 | 4.5 |
| Completeness [%] | 82.7 | 32.5 |
| Redundancy | 6.3 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PROTEIN SAMPLES AT 20 MG/ML CRYSTALLISED IN 0.1 M MES, PH 6.5, 10% W/V PWG 20000 AT ROOM TEMPERATURE. |
