4APV
The Klebsiella pneumoniae primosomal PriB protein: identification, crystal structure, and ssDNA binding mode
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2010-01-20 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 64.773, 36.934, 39.451 |
Unit cell angles | 90.00, 113.77, 90.00 |
Refinement procedure
Resolution | 26.649 - 2.095 |
R-factor | 0.2052 |
Rwork | 0.198 |
R-free | 0.27360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v1q |
RMSD bond length | 0.007 |
RMSD bond angle | 1.001 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.400 | 2.100 |
High resolution limit [Å] | 2.100 | 2.090 |
Rmerge | 0.060 | 0.260 |
Number of reflections | 5031 | |
<I/σ(I)> | 40.7 | 6.4 |
Completeness [%] | 95.6 | 99.8 |
Redundancy | 3.4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 50% ETHANOL, 10 MM SODIUM ACETATE |