4APO
AIP TPR domain in complex with human Tomm20 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-09 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 60.200, 106.820, 68.470 |
Unit cell angles | 90.00, 100.85, 90.00 |
Refinement procedure
Resolution | 28.454 - 1.895 |
R-factor | 0.1836 |
Rwork | 0.181 |
R-free | 0.23440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4aif |
RMSD bond length | 0.006 |
RMSD bond angle | 0.906 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER (FOR MR) |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.520 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.040 | 0.130 |
Number of reflections | 32055 | |
<I/σ(I)> | 18.5 | 8.7 |
Completeness [%] | 96.3 | 90.9 |
Redundancy | 3.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | PEG 3350, AMMONIUM SULFATE, BIS-TRIS PH 5.5 |