4APL
Crystal Structure of AMA1 from Neospora caninum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 251.560, 51.000, 145.340 |
| Unit cell angles | 90.00, 90.93, 90.00 |
Refinement procedure
| Resolution | 48.440 - 2.900 |
| R-factor | 0.19663 |
| Rwork | 0.193 |
| R-free | 0.27097 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x2z |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.051 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.110 | 0.380 |
| Number of reflections | 41269 | |
| <I/σ(I)> | 9.8 | 4.4 |
| Completeness [%] | 99.1 | 96.6 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG3350, 300 MM SODIUM CITRATE PH 4.2 AND 10 MM ZINC CHLORIDE |
