4AP8
Crystal structure of human Molybdopterin synthase catalytic subunit (MOCS2B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 138.968, 138.968, 159.574 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 96.090 - 2.780 |
| R-factor | 0.17296 |
| Rwork | 0.172 |
| R-free | 0.19932 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qie |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.798 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 159.570 | 2.930 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.080 | 0.800 |
| Number of reflections | 45153 | |
| <I/σ(I)> | 12.1 | 2.2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M HEPES PH 7.3, 0.8M POTASSIUM/SODIUM TARTRATE |
