4AOX
Oxidized steroid monooxygenase bound to NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-04-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 79.760, 79.760, 222.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.870 - 2.420 |
| R-factor | 0.2246 |
| Rwork | 0.223 |
| R-free | 0.25201 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w4x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.272 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.100 | 2.550 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.100 | 0.470 |
| Number of reflections | 28420 | |
| <I/σ(I)> | 11.4 | 3.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 1.9M MGSO4, 0.1M MES PH 6.2 |
