4AOS
Oxidized steroid monooxygenase bound to NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 81.560, 81.560, 224.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.310 - 2.500 |
| R-factor | 0.1988 |
| Rwork | 0.197 |
| R-free | 0.23569 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w4x |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.227 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.390 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.150 |
| Number of reflections | 26952 | |
| <I/σ(I)> | 25.1 | 14.3 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 12.6 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 1.9M MGSO4 0.1M MES PH 6.2 |
