4AOB
SAM-I riboswitch containing the T. solenopsae Kt-23 in complex with S- adenosyl methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-28 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 61.300, 61.300, 157.538 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.950 |
R-factor | 0.1914 |
Rwork | 0.189 |
R-free | 0.23943 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gx5 |
RMSD bond length | 0.016 |
RMSD bond angle | 2.545 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.060 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.110 | 0.460 |
Number of reflections | 6810 | |
<I/σ(I)> | 26.8 | 3.05 |
Completeness [%] | 98.5 | 89.5 |
Redundancy | 9.5 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 40 MM NA-CACODYLATE PH 7, 80 MM KCL, 30 MM BACL2, 12 MM SPERMINE HCL 8%(V/V) MPD |