4AO1
High resolution crystal structure of bovine pancreatic ribonuclease crystallized using ionic liquid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-19 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.006, 64.006, 63.616 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.430 - 1.580 |
| R-factor | 0.16416 |
| Rwork | 0.162 |
| R-free | 0.19558 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i67 |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.485 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.810 | 1.670 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.040 | 0.050 |
| Number of reflections | 18627 | |
| <I/σ(I)> | 26.7 | 19.2 |
| Completeness [%] | 93.7 | 98.2 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | RIBONUCLEASE A FROM BOVINE PANCREAS (SIGMA) HAS BEEN DISSOLVED IN 50 MM SODIUM ACETATE BUFFER (PH 4.57) AND CRYSTALLIZED BY THE SITTING DROP DIFFUSION METHOD |
