4ANO
Crystal Structure Geobacillus thermodenitrificans EssB cytoplasmic fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2010-02-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 74.622, 74.622, 83.545 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.610 - 1.700 |
| R-factor | 0.20777 |
| Rwork | 0.205 |
| R-free | 0.25262 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.009 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.610 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.460 |
| Number of reflections | 26751 | |
| <I/σ(I)> | 24.4 | 4.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.6 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M TRI-SODIUM CITRATE PH5.5, 20% PEG3000, 0.25MM TRIS(2-CARBOXYETHYL)PHOSPHINE HYDROCHLORIDE |
