4ANM
Complex of CK2 with a CDC7 inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 155 |
| Detector technology | CCD |
| Collection date | 2007-02-21 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.061, 60.549, 44.747 |
| Unit cell angles | 90.00, 102.92, 90.00 |
Refinement procedure
| Resolution | 36.710 - 1.700 |
| R-factor | 0.16636 |
| Rwork | 0.164 |
| R-free | 0.20184 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE CK2 APO STRUCTURE |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.373 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.710 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.030 | 0.130 |
| Number of reflections | 40017 | |
| <I/σ(I)> | 31.58 | 7.9 |
| Completeness [%] | 97.9 | 91.8 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 20% PEG4K, 200 MM NAOAC, 100 MM TRIS, PH 8.0 |
