4ANI

Structural basis for the intermolecular communication between DnaK and GrpE in the DnaK chaperone system from Geobacillus kaustophilus HTA426

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	273
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.02249, 1.03964
Spacegroup name	I 41 2 2
Unit cell lengths	279.975, 279.975, 278.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.221 - 4.094
R-factor	0.2773
Rwork	0.274
R-free	0.34740
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.022
RMSD bond angle	1.840
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.220	4.350
High resolution limit [Å]	4.090	4.100
Rmerge	0.090	0.490
Number of reflections	41248
<I/σ(I)>	32.3	2.8
Completeness [%]	94.8	94.5
Redundancy	8.5	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1