4ANG
Small RNA phage PRR1 in complex with an RNA operator fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 281.300, 285.400, 472.900 |
| Unit cell angles | 93.23, 90.03, 119.45 |
Refinement procedure
| Resolution | 29.980 - 3.500 |
| R-factor | 0.308 |
| Rwork | 0.308 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vf9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.130 | 0.390 |
| Number of reflections | 259990 | |
| Completeness [%] | 16.0 | 15 |
| Redundancy | 1.24 | 1.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
