4AN4
Crystal structure of the glycosyltransferase SnogD from Streptomyces nogalater
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 64.641, 70.078, 176.031 |
| Unit cell angles | 90.00, 91.71, 90.00 |
Refinement procedure
| Resolution | 60.000 - 2.700 |
| R-factor | 0.22947 |
| Rwork | 0.227 |
| R-free | 0.26595 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4amb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.428 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.600 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 42845 | |
| <I/σ(I)> | 17.7 | 2.2 |
| Completeness [%] | 98.3 | 92.7 |
| Redundancy | 4.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 15 % (W/V) PEG 8000 0.16 M CALCIUM ACETATE 0.08 M SODIUM CACODYLATE PH 6.8 |
