4AMU

Structure of ornithine carbamoyltransferase from Mycoplasma penetrans with a P321 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 3 2 1
Unit cell lengths	183.648, 183.648, 117.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.425 - 2.500
R-factor	0.1724
Rwork	0.170
R-free	0.21510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.154
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.420	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.090	0.440
Number of reflections	77424
<I/σ(I)>	12.1	2.6
Completeness [%]	98.2	89.7
Redundancy	4.8	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1