4AMS
A Megaviridae ORFan gene encode a new nucleotidyl transferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2011-11-18 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 100.632, 100.632, 168.822 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.558 - 2.600 |
R-factor | 0.213 |
Rwork | 0.211 |
R-free | 0.24680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4amq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.338 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CaspR |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.600 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.090 | 0.480 |
Number of reflections | 26030 | |
<I/σ(I)> | 6.3 | |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 8.2 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | PROTEIN CONCENTRATION: 5 TO 12 MG/ML IN 10 MM CAPS BUFFER, 150 MM NACL, PH 10.5 RESERVOIR SOLUTION: 150 MM NA ACETATE PH 6 |