4AMQ
A Megaviridae Orfan gene encodes a new nucleotidyl transferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2010-11-07 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.445, 91.092, 152.994 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.290 - 2.170 |
R-factor | 0.2206 |
Rwork | 0.219 |
R-free | 0.24420 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.003 |
RMSD bond angle | 0.678 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | autoSHARP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.400 | 2.250 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.080 | 0.360 |
Number of reflections | 20963 | |
<I/σ(I)> | 3.8 | 1.9 |
Completeness [%] | 96.0 | 96 |
Redundancy | 2.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | PROTEIN CONCENTRATION: 10 MG/ML IN 10 MM CHES BUFFER PH 9.0 RESERVOIR: 4 TO 12% PEG 4000, 0.1 M SODIUM CACODYLATE BETWEEN PH 6 AND 7, 0.1 M MNCL2 |