4AMC
Crystal structure of Lactobacillus reuteri 121 N-terminally truncated glucansucrase GTFA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 183.871, 183.871, 374.004 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.600 |
| R-factor | 0.2399 |
| Rwork | 0.239 |
| R-free | 0.25972 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3klk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.763 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 3.790 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.220 | 0.950 |
| Number of reflections | 44037 | |
| <I/σ(I)> | 6.7 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.5 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 1 M (NH4)2SO4, 0.1 M SODIUM PHOSPHATE-CITRATE BUFFER PH 4.2 |
