4ALZ
The Yersinia T3SS basal body component YscD reveals a different structural periplasmatic domain organization to known homologue PrgH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-16 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.990, 51.550, 50.630 |
Unit cell angles | 90.00, 106.08, 90.00 |
Refinement procedure
Resolution | 29.791 - 1.400 |
R-factor | 0.1912 |
Rwork | 0.189 |
R-free | 0.22580 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.009 |
RMSD bond angle | 1.238 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.800 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.040 | 0.550 |
Number of reflections | 36930 | |
<I/σ(I)> | 21 | 2.3 |
Completeness [%] | 95.5 | 99.7 |
Redundancy | 3.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | YSCD150-347 G283P CRYSTALS GREW FROM EQUAL VOLUMES OF PROTEIN (5.8 MG/ML IN 20 MM HEPES PH 7.0, 60 MM NACL) WITH PRECIPITANT SOLUTION (0.15 M NAH2PO4, 20 % (W/V) PEG 3350, 60 MM NACL) IN HANGING-DROP VAPOR-DIFFUSION CRYSTALLIZATION TRAYS AT 292 K (EASYXTAL; QIAGEN). |