4AL6
Crystal structure of the S148ACsy4-crRNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-02 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.390, 46.880, 87.390 |
| Unit cell angles | 90.00, 107.24, 90.00 |
Refinement procedure
| Resolution | 41.733 - 2.630 |
| R-factor | 0.2031 |
| Rwork | 0.200 |
| R-free | 0.25210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4al5 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.477 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.730 | 2.700 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.100 | 0.650 |
| Number of reflections | 8517 | |
| <I/σ(I)> | 13.44 | 2.54 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 20% PEG 4000, 150 MM NA CITRATE PH 5.0, 100 MM MGCL2 |
