4AKX
Structure of the heterodimeric complex ExoU-SpcU from the type III secretion system (T3SS) of Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.887, 52.799, 121.811 |
| Unit cell angles | 90.00, 127.18, 90.00 |
Refinement procedure
| Resolution | 97.050 - 2.940 |
| R-factor | 0.21102 |
| Rwork | 0.208 |
| R-free | 0.26437 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.291 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.050 | 3.120 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Rmerge | 0.090 | 0.670 |
| Number of reflections | 17008 | |
| <I/σ(I)> | 13.1 | 3 |
| Completeness [%] | 98.1 | 91.6 |
| Redundancy | 6.4 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 50 MM BICINE PH 9.0, 10% PEG 4000 |
