4AKP
Mutations in the neighbourhood of CotA-laccase trinuclear site: E498T mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-20 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.898, 101.898, 136.931 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.110 - 2.000 |
| R-factor | 0.17863 |
| Rwork | 0.177 |
| R-free | 0.21497 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w6l |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.143 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.390 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.350 |
| Number of reflections | 55879 | |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 5.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 8 % PEG 4K, 0.1 M SODIUM CITRATE PH5.5, 26 % ISOPROPANOL |
