4AKK
Structure of the NasR transcription antiterminator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-05 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.230, 113.100, 59.350 |
| Unit cell angles | 90.00, 109.30, 90.00 |
Refinement procedure
| Resolution | 43.866 - 2.145 |
| R-factor | 0.2025 |
| Rwork | 0.200 |
| R-free | 0.24130 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.856 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 53696 | |
| <I/σ(I)> | 9.79 | 2.5 |
| Completeness [%] | 98.7 | 93.1 |
| Redundancy | 3.95 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES PH7.5, 20 MM MGCL2, 22 % POLYACRYLIC ACID, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
