4AKE
ADENYLATE KINASE
Experimental procedure
| Source type | ROTATING ANODE |
| Detector technology | AREA DETECTOR |
| Collection date | 1991 |
| Detector | SIEMENS |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.800, 54.500, 71.300 |
| Unit cell angles | 67.70, 77.90, 88.40 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.063 |
| Number of reflections | 17932 |
| Completeness [%] | 82.0 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | MOPS | 50 (mM) | pH6.9 |
| 3 | 1 | drop | PEG6000 | 13-18 (%) | |
| 4 | 1 | drop | 0.02 (%) | ||
| 5 | 1 | reservoir | PEG6000 | 23-27 (%) | |
| 6 | 1 | reservoir | MOPS | 50 (mM) | pH6.9 |
