4AK0
Ligand controlled assembly of hexamers, dihexamers, and linear multihexamer structures by an engineered acylated insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-06 |
| Detector | MARRESEARCH |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 77.800, 77.800, 77.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.050 - 2.280 |
| R-factor | 0.17263 |
| Rwork | 0.166 |
| R-free | 0.23165 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b2f |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.802 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 2.420 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.120 | 0.380 |
| Number of reflections | 3406 | |
| <I/σ(I)> | 18.4 | 7 |
| Completeness [%] | 91.9 | 100 |
| Redundancy | 12.1 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | pH 7.8 |
