4AJX
Ligand controlled assembly of hexamers, dihexamers, and linear multihexamer structures by an engineered acylated insulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.310, 62.490, 57.270 |
Unit cell angles | 90.00, 111.80, 90.00 |
Refinement procedure
Resolution | 40.497 - 1.200 |
R-factor | 0.1415 |
Rwork | 0.141 |
R-free | 0.15960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1evr |
RMSD bond length | 0.014 |
RMSD bond angle | 1.614 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE: DEV_947)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.500 | 1.230 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.036 | 0.410 |
Number of reflections | 90966 | |
<I/σ(I)> | 19.15 | 2.51 |
Completeness [%] | 92.4 | 60.3 |
Redundancy | 4.2 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.6 M IMIDAZOLE/MALONIC ACID PH 7.0 |