4AJV
Structure of mouse ZP-C domain of TGF-Beta-Receptor-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.660, 56.820, 60.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.318 - 2.700 |
| R-factor | 0.2242 |
| Rwork | 0.220 |
| R-free | 0.29580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qw9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.753 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.320 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.100 | 0.640 |
| Number of reflections | 4960 | |
| <I/σ(I)> | 11.2 | 2.3 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M HEPES PH 6 TO 7, 0.2 M AMMONIUM ACETATE, 20 TO 30 % PEG 3350 |
