4AJ8
Crystallographic structure of thioredoxin from Litopenaeus vannamei (partially reduced).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-11 |
| Detector | RIGAKU R-AXIS IV PLUS PLUS |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 57.523, 57.523, 118.016 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.218 - 1.540 |
| R-factor | 0.1802 |
| Rwork | 0.178 |
| R-free | 0.21520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zzx |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.353 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.370 | 1.620 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.060 | 0.250 |
| Number of reflections | 33397 | |
| <I/σ(I)> | 6.6 | 3 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 6.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 2.0 M AMMONIUM SUPHATE, 0.1 M SODIUM ACETATE, PH 4.6 |
