4AJ7
Crystallographic structure of thioredoxin from Litopenaeus vannamei (oxidized form).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-08 |
Detector | ADSC CCD |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 57.895, 57.895, 117.869 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.094 - 2.035 |
R-factor | 0.1768 |
Rwork | 0.174 |
R-free | 0.22490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zzx |
RMSD bond length | 0.009 |
RMSD bond angle | 1.066 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.640 | 2.150 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.060 | 0.240 |
Number of reflections | 14756 | |
<I/σ(I)> | 3.1 | 3.1 |
Completeness [%] | 99.4 | 96.6 |
Redundancy | 6.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 2.0 M AMMONIUM SUPHATE, 0.1 M SODIUM ACETATE, PH 4.6 |