4AJ0
Crystallographic structure of an amyloidogenic variant, 3rCW, of the germinal line lambda 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-01 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 31 |
Unit cell lengths | 41.807, 41.807, 171.035 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.631 - 1.700 |
R-factor | 0.1765 |
Rwork | 0.174 |
R-free | 0.21160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lil |
RMSD bond length | 0.006 |
RMSD bond angle | 1.135 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.060 | 0.430 |
Number of reflections | 36831 | |
<I/σ(I)> | 10.2 | 1.8 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 5.7 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 3 | 1.6 M AMMONIUM SULFATE, 40 % TREHALOSE, pH 3 |