4AIZ
Crystallographic structure of 3mJL2 from the germinal line lambda 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-01 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.402, 40.946, 106.726 |
| Unit cell angles | 90.00, 90.15, 90.00 |
Refinement procedure
| Resolution | 34.224 - 1.750 |
| R-factor | 0.1689 |
| Rwork | 0.166 |
| R-free | 0.21850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lil |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.056 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.370 |
| Number of reflections | 31323 | |
| <I/σ(I)> | 7.4 | 2.1 |
| Completeness [%] | 79.4 | 74.3 |
| Redundancy | 6.8 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M SODIUM CITRATE, 0.1 M HEPES, 30 % MPD, pH 7.5 |
