4AIW
GAPR-1 with bound inositol hexakisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.088, 64.872, 103.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.460 - 1.500 |
| R-factor | 0.15422 |
| Rwork | 0.152 |
| R-free | 0.18736 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1smb |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.834 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.040 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.430 |
| Number of reflections | 21759 | |
| <I/σ(I)> | 9.9 | 3.4 |
| Completeness [%] | 91.8 | 94.7 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% PEG 3350, 100 MM MMT, PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
