4AIR
Leishmania major cysteine synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-17 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.958, 86.325, 134.019 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 1.800 |
R-factor | 0.15921 |
Rwork | 0.157 |
R-free | 0.20826 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bhs |
RMSD bond length | 0.015 |
RMSD bond angle | 1.438 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.900 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.070 | 0.490 |
Number of reflections | 53553 | |
<I/σ(I)> | 18 | 3.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |