4AIM
Crystal structure of C. crescentus PNPase bound to RNase E recognition peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-14 |
| Detector | MARRESEARCH MX-300 |
| Spacegroup name | P 63 |
| Unit cell lengths | 97.329, 97.329, 191.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.730 - 3.300 |
| R-factor | 0.18927 |
| Rwork | 0.186 |
| R-free | 0.26026 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gme |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.703 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.140 | 0.650 |
| Number of reflections | 15391 | |
| <I/σ(I)> | 7.8 | 2.4 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 17 % WT/V PEG 3000, 0.1 M TRI-SODIUM CITRATE |
