4AIJ
Crystal structure of RovA from Yersinia in complex with a rovA promoter fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-28 |
Detector | ADSC CCD |
Spacegroup name | P 3 |
Unit cell lengths | 88.779, 88.779, 67.729 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 76.900 - 2.050 |
R-factor | 0.20562 |
Rwork | 0.203 |
R-free | 0.25132 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3deu |
RMSD bond length | 0.013 |
RMSD bond angle | 1.664 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.010 | 0.050 |
Number of reflections | 39678 | |
<I/σ(I)> | 15.3 | 2 |
Completeness [%] | 97.6 | 89 |
Redundancy | 5.3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 10 MM MGCL2, 50 MM MES PH 5.6, 2.3 M LISO4 |