4AII
Crystal structure of the rat REM2 GTPase - G domain bound to GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 287 |
| Detector technology | PIXEL |
| Collection date | 2011-10-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.916, 59.374, 57.297 |
| Unit cell angles | 90.00, 101.51, 90.00 |
Refinement procedure
| Resolution | 41.650 - 2.660 |
| R-factor | 0.24693 |
| Rwork | 0.243 |
| R-free | 0.32436 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cbq |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.633 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.650 | 2.800 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.110 | 0.410 |
| Number of reflections | 9524 | |
| <I/σ(I)> | 5.7 | 2 |
| Completeness [%] | 98.8 | 98.8 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 13.5 % PEG 4000, 10% ISOPROPANOL, 100 MM HEPES SODIUM SALT PH 7.4 |
