4AHX
Flo5A showing a trinuclear gadolinium cluster on its surface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-03-16 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.230, 62.150, 105.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.550 - 1.600 |
| R-factor | 0.15358 |
| Rwork | 0.153 |
| R-free | 0.18327 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xjp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.463 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.290 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.050 | 0.380 |
| Number of reflections | 44903 | |
| <I/σ(I)> | 16.9 | 2.9 |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 50 MM BISTRIS PROPANE, PH 7.8, 500 MM NACL, 20% PEG 4000 |
