4AH4
Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) in complex with BGA Oligosaccharide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.420, 47.440, 80.086 |
| Unit cell angles | 103.61, 91.96, 113.08 |
Refinement procedure
| Resolution | 77.150 - 1.750 |
| R-factor | 0.16978 |
| Rwork | 0.168 |
| R-free | 0.21075 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4agi |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.688 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.030 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.050 | 0.350 |
| Number of reflections | 57394 | |
| <I/σ(I)> | 14.3 | 2.6 |
| Completeness [%] | 94.1 | 85.3 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM CACL2, 25% PEG 4K AND 100 MM TRIS, PH 8.5 BGA OLIGOSACCHARIDE |
